mouse monoclonal anti-glua1  (Santa Cruz Biotechnology)

Journal: bioRxiv

Article Title: Granulocyte colony-stimulating factor acts through calcium-permeable AMPA receptors to potentiate cocaine reward

doi: 10.64898/2026.01.30.702629

Figure Lengend Snippet: A.Percent change PSD95 expression from saline (control) in mice treated with G-CSF (50 ug/kg), cocaine (7.5 mg/kg), or G-CSF+cocaine (50 ug/kg + 7.5 mg/kg). B . Percent change GluR1 expression from saline (control) in mice treated with G-CSF, cocaine, or G-CSF+cocaine. C . Percent change GluR2 expression from saline (control) in mice treated with G-CSF, cocaine, or G-CSF+cocaine. D . Example PSD95 blot. E . Example GluR1 blot. F . Example GluR2 blot. * p < 0.05.

Article Snippet: Proteins were transferred to a PVDF membrane and blocked with Intercept blocking buffer (LiCor) before incubation with GluR1 (1:1000, Cell Signaling Technology #13185), PSD95 (1:1000, Cell Signaling Technology #36233), or GluR2 (1:1000, Cell Signaling Technology #13607), and actin (1:5000, MP Biomedicals #691001) antibodies overnight at 4°C.

Techniques: Expressing, Saline, Control

Journal: bioRxiv

Article Title: Disruption of hippocampal mitochondrial function underlies opioid-induced postoperative cognitive dysfunction in aged rats

doi: 10.64898/2026.01.30.702943

Figure Lengend Snippet: Synaptic plasticity, circulating neurofilament light chain, and glutamate receptor subunit expression. (A) Time course of field excitatory postsynaptic potential (fEPSP) response expressed as mean peak fEPSP (% baseline) following theta burst stimulation (TBS) in hippocampal slices from saline- and morphine-treated rats. ***p < 0.001 (B) Circulating neurofilament light chain (Nf-L) concentrations measured 30 days post-surgery in saline- and morphine-treated rats. Data are presented as mean ± SEM with individual data points shown. *p < 0.05. (C-G) Protein expression of glutamate receptor subunits GluA1 (C), GluA2/3 (D), GluN1 (E), GluN2a (F), and GluN2b (G) measured in hippocampal synaptoneurosomes under baseline conditions and following a learning experience, expressed as percent of laparotomy saline baseline control. (H) Representative immunoblots for glutamate receptor subunits and GAPDH corresponding to quantified data shown in panels C-G. Data are presented as mean ± SEM with individual data points shown. *p < 0.05.

Article Snippet: Primary antibodies, in Odyssey blocking solution containing 0.2% Tween20 (overnight at 4°C), were: SYP (1:1,000; sc-17750; Santa Cruz), PSD95 (1:1,000; 2507S; Cell Signaling), GluA1 (1:1000; 13185S; Cell Signaling) GluA2/3 (1:1000, 07-598; Millipore Sigma), GluN1 (1:500; MAB1586; Millipore Sigma), GluN2A (1:500; 07-632; Millipore Sigma) and GluN2B (1:1000; ab254356; Abcam),

Techniques: Expressing, Saline, Control, Western Blot